More Articles

• Yeast That Smell
  • Eugenia Y Xu, Addison D Ault, James R Broach*
• A possible role of repair proteins BRCA1 and DNA-PK in the processing of oxidative DNA damage
  • Alexandros G Georgakilas
• How effective is T-DNA insertional mutagenesis in Arabidopsis?
  • Yi Hong Wang
• Hatching enzyme of Volvox: a possible implication in the evolution of multicellularity
  • Hideaki Shiraish

Special Contributions

• In search of best impact factor and citation indexed journals towards achieving the goals of universities
  • Hj. Kamaruzaman Jusoff
• Recent abstracts in biochemical technology
  • R R Siva Kiran, Brijesh

Department of Biochemical Engineering, Zhejiang University of Technology, Zhejiang, China

Bioimmobilization with sol-gel method is a complicate system,
involving many parameters that can affect sol-gel structure and
immobilized biomolecules activity. In this study, trypsin, a model
enzyme, was entrapped into sol-gel glasses produced from
tetraethoxysilane (TEOS) and water to study the relationships
between the immobilization parameters, namely ratio of water to
TEOS, volume of poly (ethylene glycol) (PEG), concentration of
buffer solution, pH of buffer solution by using statistical methods.
Two regression equations and analysis of variance (ANOVA) were
obtained with the entrapped enzyme activity and enzyme loading
yield as the response values, respectively. Statistical calculations
showed that the effects of the four variables on the entrapped
enzyme activity and enzyme loading yield were different. Entrapped
trypsin activity (35.6%) and trypsin loading yield (68.3%) were
obtained by using optimized immobilization conditions. The results
of this study may help to manipulate the properties of sol-gel to get
high immobilization efficiency.