Determinant factors for hybridmyeloma culture (H9r9) in the yield of anti - D – impact of medium (IMDM and RPMI), serum (FCS) and cell density

J Biochem Tech

Volume 1, Issue 2, December 2008, Page No. 53-56

Determinant factors for hybridmyeloma culture (H9r9) in the yield of anti - D – impact of medium (IMDM and RPMI), serum (FCS) and cell density

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Sathya R, Mathialagan A, Flora Bai B, Ramalingam K

Mediclone Research Centre, Mediclone Biotech Pvt.Ltd, Velichai, Kolathur Post, Chennai – 600 048, India

Received: 12 January 2009 / Received in revised form: 6 February 2009, Accepted: 25 February 2009, Published online: 28 February 2009

Abstract

The hybridoma (H9r9) were cultured in roller bottles with two media
viz IMDM and RPMI with FCS (3% & 5%). The parameters such as
cell count, cell viability and avidity were determined on a time
course basis from day 1 to day 18, to assess the optimization of the
roller bottle process for the production of anti – D and to determine
the influence of factors namely medium, serum concentration and
varying cell density. The cell viability and cell count remained
significant upto 8 or 10 days. Cell viability assessed by dye binding
revealed that apoptosis of hybridoma cells sets suddenly after 10
days in all culture bottles. The observations implied that 0.2 10⁶/
ml seemed to be optimum for growth of hybridoma. IMDM seemed
to be comparatively superior to RPMI. The study also warrants
empirical informations of H9r9 cell metabolism to achieve
optimization.

Keywords: Hybridoma (H9r9), Foetal calf serum (FCS), Anti – D,
Apoptosis, IMDM/RPMI Medium

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J Biochem Tech

Volume 1, Issue 2, December 2008, Page No. 53-56




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