Effects of leucine-enkephalin on alpha-naphthyl acetate esterase and myeloperoxidase activity in haemolymph of the scallop Chlamys farreri

J Biochem Tech

Volume 1, Issue 2, December 2008, Page No. 45-48

Effects of leucine-enkephalin on alpha-naphthyl acetate esterase and myeloperoxidase activity in haemolymph of the scallop Chlamys farreri

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Dongwu Liu

Analysis and Testing Center, Shandong University of Technology, 255049, Zibo, Shandong, P.R. China

Received: 23 September 2008 / Received in revised form: 6 October 2008, Accepted: 7 October 2008 Published online: 18 October 2008

Abstract

The study was designed to investigate the effects of leucineenkephalin
(L-ENK) on the activity of alpha-naphthyl acetate
esterase (ANAE) and myeloperoxidase (MPO) in haemolymph of
the scallop Chlamys farreri. Our results indicate that MPO activity
with DAB and DEA substrate solution was induced when the
concentration of L-ENK was 5 and 50 μg/ml, and the intracellular
and extracellular MPO activity with two substrate solutions was
highest when the concentration of L-ENK was 50 μg/ml. However,
both the intracellular and extracellular MPO activity of 1 μg/ml
group was significantly lower than that of control group. It was also
found that the intracellular and extracellular ANAE activity was
induced by L-ENK. The supernatant ANAE activity was highest
when the concentration of L-ENK was 50 μg/ml, but the resultant
HLS ANAE activity was highest when the concentration of L-ENK
was 5 μg/ml. Overall, the data suggests an involvement of L-ENK in
the regulation of the defense systems of the scallop Chlamys farreri.
L-ENK may function as messengers in the bidirectional interaction
between the neuroendocrine and the immune systems.

Keywords: Chlamys farreri; Leucine-enkephalin; Alpha-naphthyl
acetate esterase; Myeloperoxidase; Haemolymph

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J Biochem Tech

Volume 1, Issue 2, December 2008, Page No. 45-48




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